Running Title : ExoU and ExoY induce endothelial proteinopathy 1 1 2 Pseudomonas aeruginosa exoenzymes U and Y induce a 3 transmissible endothelial proteinopathy 4 5
نویسندگان
چکیده
K. Adam Morrow, Cristhiaan D. Ochoa, Ron Balczon, Chun Zhou, Laura Cauthen, 6 Mikhail Alexeyev, Katherine M. Schmalzer, Dara W. Frank, and Troy Stevens 7 8 Departments of Physiology and Cell Biology, Biochemistry and Molecular Biology, and 9 Medicine, Center for Lung Biology, University of South Alabama, Mobile AL 36688; Physician10 Scientist Training Program, Department of Medicine and Division of Pulmonary and Critical 11 Care, University of Texas-Southwestern Medical Center, Dallas TX 75390; Department of 12 Medicine, Division of Hematology/Oncology, Department of Microbiology and Molecular 13 Genetics, Center for Infectious Disease Research, Medical College of Wisconsin, Milwaukee 14 WI 53226 15 16 17
منابع مشابه
Pseudomonas aeruginosa exoenzymes U and Y induce a transmissible endothelial proteinopathy.
We tested the hypothesis that Pseudomonas aeruginosa type 3 secretion system effectors exoenzymes Y and U (ExoY and ExoU) induce release of a high-molecular-weight endothelial tau, causing transmissible cell injury characteristic of an infectious proteinopathy. Both the bacterial delivery of ExoY and ExoU and the conditional expression of an activity-attenuated ExoU induced time-dependent pulmo...
متن کاملHeterogeneity of pulmonary endothelial cyclic nucleotide response to Pseudomonas aeruginosa ExoY infection.
Here, we tested the hypothesis that a promiscuous bacterial cyclase synthesizes purine and pyrimidine cyclic nucleotides in the pulmonary endothelium. To test this hypothesis, pulmonary endothelial cells were infected with a strain of the Gram-negative bacterium Pseudomonas aeruginosa that introduces only exoenzyme Y (PA103 ΔexoUexoT::Tc pUCPexoY; ExoY(+)) via a type III secretion system. Purin...
متن کاملThe Pseudomonas aeruginosa exoenzyme Y impairs endothelial cell proliferation and vascular repair following lung injury.
Exoenzyme Y (ExoY) is a Pseudomonas aeruginosa toxin that is introduced into host cells through the type 3 secretion system (T3SS). Once inside the host cell cytoplasm, ExoY generates cyclic nucleotides that cause tau phosphorylation and microtubule breakdown. Microtubule breakdown causes interendothelial cell gap formation and tissue edema. Although ExoY transiently induces interendothelial ce...
متن کاملThe Frequency of Exotoxin A and Exoenzymes S and U Genes Among Clinical Isolates of Pseudomonas aeruginosa in Shiraz, Iran
Pseudomonas aeruginosa as an opportunistic pathogen produces several virulence factors. The most important of these factors are exotoxin A and type III secretion system (T3SS). The aim of this study was to determine the frequency of toxA, exoU and exoS genes among clinical isolates of P. aeruginosa. In this cross-sectional study from September 2011 to February 2012, 156 P. aeruginosa isolates w...
متن کاملPseudomonas aeruginosa infection of airway epithelial cells modulates expression of Kruppel-like factors 2 and 6 via RsmA-mediated regulation of type III exoenzymes S and Y.
Pseudomonas aeruginosa is an important opportunistic pathogen which is capable of causing both acute and chronic infections in immunocompromised patients. Successful adaptation of the bacterium to its host environment relies on the ability of the organism to tightly regulate gene expression. RsmA, a small RNA-binding protein, controls the expression of a large number of virulence-related genes ...
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تاریخ انتشار 2015